Lead is causing severe pollution. It has a negative impact on normal agricultural and forestry production, and crops contaminated by heavy metals can lead to diseases that threaten human’s health. No obvious solution has yet been proposed. Our project is designed to solve this issue by extracting the PtoMYB46, sequence of genes that is proved to have the function of absorbing the lead ions in previous detection, and converting it into poplars’ genes. We firstly extracted genes from the poplars' DNA and amplified the target genes using PCR technique after the detection of its purity and concentration. The cloning vector and expressing vectors were constructed in the second step. Finally, we transferred the gene to the agrobacteria to infect the poplars' leaves. The transgenic poplar improved and perfected the function of absorbing lead ions in poplar. We use directed evolution to further perfect tne function of genes.