Team:OUC-China
fermentation industries
facing a super crisis:
strain degradation
A large number of engineering bacteria robots in the Fermentation Kingdom have degenerated into useless zombie robots that do not work.
"Dispers" is the embodiment of our OUC team and form a rescue squad carries a DISP(Directional intelligent screening platform) magic tool to solve the problem of strain degradation.
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Dispers
The embodiment of our OUC team.
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DISP
Magic tool to solve the degradation of strain: Directional Intelligent Screening Platform.
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CHIP chip
Produce a specific solution for a specific fermentation kingdom.
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Rebrith
Strain degradation problem solved.
Introducing
CHIP chip
The chip consists of four parts:
1) control 2) hold 3) inhibition and 4) transport
Introduction of the four parts
Part 1：CONTROL——quorum sensing
To increase the competitiveness with wild-type, we build an quorum sensing system which can up or down regulate the SSRE-promoter depending on the con-centration of IP. The engineering bacteria can only produce when the it reaches a certain level.
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Part 2：HOLD——weak promoter
To keep the plasmid copy-number at high level in cell. we knockout an essential gene and insert it after a weak-expressed promoter. The strain will die when the copy number decrease to a certain level.
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Part 3：INHIBITION——kill switch
To prevent our strains from escaping into the environment, and as a mean for us to control production. We built an riboswitch which is regulated by an specific aptamer, the YopE will be transcribed to kill the strain when the production is low enough.
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Part 4：TRANSPORT——transporter
In order to solve the problem that the production cannot be transported to extracellular, we insert the binding domain of the production-aptamer to an aptamer that can target to membrane to transport the production specifically.
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