Recipe: BG-11 Medium
Medium for cultivation of Cyanobacteria
To make BG-11 media, you need to prepare various stock solutions. The recipes for the particular solutions you find in the table below. Stock solutions 1-3 need to be sterile filtered or autoclaved.
Stock Solution 1
| # |
Component Name |
Amount |
| 1 |
Na2MG EDTA |
0.1 g/liter |
| 2 |
Ferric ammonium citrate |
0.6 g/liter |
| 3 |
Citric acid 1H2O |
0.6 g/liter |
| 4 |
CaCl2 2H2O |
3.6 g/liter |
Stock Solution 2
| # |
Component Name |
Amount |
| 1 |
MgSO4 7H2O |
7.5 g/liter |
Stock Solution 3
| # |
Component Name |
Amount |
| 1 |
K2HPO4 3H2O |
4.0 g/liter |
| 2 |
OR alternatively K2HPO4 |
3.05 g/liter |
Stock Solution 5
| # |
Component Name |
Amount |
| 1 |
H3BO3 |
2.86 g/liter |
| 2 |
MnCl2 4H2O |
1.81 g/liter |
| 3 |
ZnSO4 7H2O |
0.222 g/liter |
| 4 |
CuSO4 5H2O |
0.079 g/liter |
| 5 |
COCl2 6H2O |
0.050 g/liter |
| 6 |
NaMoO4 2H2O |
0.391 g/liter |
| 6 alt. |
alternative: MoO4 (85%) |
0.018 g/liter |
For the basic BG11 medium, combine the following stock solutions:
Recipe BG-11 medium
| # |
Stock Solution |
Per Liter of Medium |
| 1 |
Stock 1 |
10 ml |
| 2 |
Stock 2 |
10 ml |
| 3 |
Stock 3 |
10 ml |
| 4 |
Na2CO3 |
0.02 g |
| 5 |
Stock 5 |
1.0 ml |
| 6 |
NaNO3 |
1.5 g |
Combine stocks and adjust the pH to 7.5 (with 1.0N HCl). Autoclave.