CHLAMYDOMON[As]
CHLAMYDOMON[As]
The Solution: Chlamydomon[As]
Read more here, on our Project Description page.
Proposed Implementation
Our solution is designed to exist within a larger water filtration system to remove all contaminants and provide potable water to rural communities. We’ve designed two versions of water purification systems that are adaptable to the community’s needs.
Multicistronic Gene Expression in C. Reinhardtii with 2A Peptides
Engineering the C. reinhardtii nucleus to accept a multicistronic plasmid involves the use of 2A self-cleaving peptides, of which only a handful have been characterized for use in Chlamydomonas. We tested a total of 6 different 2A peptides to determine which had the highest cleaving efficiency, and developed a template plasmid that can accommodate up to three genes of interest using this 2A peptide system for future teams to use.
For more information on this, click here to be redirected to our Proposed Implementation page.
Human Practices
Our team engaged in several conversations with civic, industry, and academic stakeholders in order to gain insight into the deeper context surrounding our project and its aims. The insights we gained from these conversations directly informed the design and scope of our project.
Want to know more about our integrated Human Practice efforts? Click here!
iGEM Phototroph Community 2022
We partnered with UBCO iGEM to carry on the iGEM Phototroph Community started by the Marburg and Bielefeld teams in 2021 to continue giving iGEM teams working with phototrophic chassis a platform to connect and get tailored insights on their projects. We also created Volume II of the Phototroph Community handbook, now including more information on cloning and transformation, detailed protocols, and video tutorials to further assist future teams working with phototrophs.